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Nvidia
Nvidia Clara Parabricks fq2bam
Container
Nvidia
Nvidia Clara Parabricks fq2bam

Generate BAM/CRAM output given one or more pairs of FASTQ files. Can also optionally generate a BQSR report.

fq2bam performs the following steps:

The user can decide to turn-off marking of duplicates. The BQSR step is only performed if the --knownSites input and --out-recal-file output options are provided.

For further information visit the fq2bam help page.

Quick Start

# This command assumes all the inputs are in <INPUT_DIR> and all the outputs go to <OUTPUT_DIR>.
$ docker run --rm --gpus all --volume <INPUT_DIR>:/workdir --volume <OUTPUT_DIR>:/outputdir
    -w /workdir \
    nvcr.io/nvidia/clara/clara-parabricks:<VERSION-TAG> \
    pbrun fq2bam \
    --ref /workdir/${REFERENCE_FILE} \
    --in-fq /workdir/${INPUT_FASTQ_1} /workdir/${INPUT_FASTQ_2}  \
    --knownSites /workdir/${KNOWN_SITES_FILE} \
    --out-bam /outputdir/${OUTPUT_BAM} \
    --out-recal-file /outputdir/${OUTPUT_RECAL_FILE}
Publisher
Nvidia
Latest Tag4.0.0-1
UpdatedSeptember 27, 2022 UTC
Compressed Size92.85 MB
Multinode SupportNo
Multi-Arch SupportNo

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